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Using stem cell-derived heart muscle cells to advance heart regenerative therapy
利用干细胞源性心肌细胞推进心脏再生治疗

Regenerative heart therapies involve transplanting cardiac muscle cells into damaged areas of the heart to recover lost function. However, the risk of arrhythmias following this procedure is reportedly high.
再生心脏疗法包括将心肌细胞移植到心脏受损区域,以恢复失去的功能。然而,据报道,手术后心律失常的风险很高。

Xi Ruihan

Overview 概述


Regenerative heart therapies involve transplanting cardiac muscle cells into damaged areas of the heart to recover lost function. However, the risk of arrhythmias following this procedure is reportedly high. In a recent study, researchers tested a novel approach that involves injecting 'cardiac spheroids,' cultured from human stem cells, directly into damaged ventricles. The highly positive outcomes observed in primate models highlight the potential of this strategy.


再生心脏疗法包括将心肌细胞移植到心脏受损区域,以恢复失去的功能。然而,据报道,手术后心律失常的风险很高。在最近的一项研究中,日本研究人员测试了一种新方法,包括将从人类干细胞培养的“心脏球体”直接注射到受损的心室。在灵长类动物模型中观察到的高度积极的结果凸显了这一策略的潜力。


Research Subject 研究主题


The use of hiPSCs for regenerative heart therapy has been a widespread concern. HIPSCs are cells derived from mature cells that can be effectively "reprogrammed" into completely different cell types, some lost function can be restored by transplanting or injecting cardiomyocytes derived from hiPSCs into damaged areas of the heart. However, this approach increases the risk of cardiac arrhythmias and poses a major obstacle to clinical trials. A Japanese team recently tested a new strategy for regenerative heart therapy.


使用HiPSCs进行再生心脏治疗一直被广泛关注。HiPSCs是来源于成熟细胞的细胞,可以有效地“重编程”成完全不同的细胞类型,通过将源自HiPSCs的心肌细胞移植或注射到心脏的受损区域,可以恢复一些失去的功能。但是这种方法会增加心律失常的风险,对临床试验构成主要障碍。最近一个日本研究小组测试了一种再生心脏治疗的新策略。


Analysis of Research Ideas 研究思路分析

Receptor-based Signaling

In their novel approach, the researchers cultivated HiPSCs in a medium that led to their differentiation into cardiomyocytes. After carefully extracting and purifying cardiac spheroids (three-dimensional clusters of cardiac cells) from the cultures, they injected approximately 6 × 10⁷ cells into the damaged hearts of crab-eating macaques (Macaca fascicularis). They monitored the condition of the animals for twelve weeks, taking regular measurements of cardiac function. Following this, they analyzed the monkeys' hearts at the tissue level to assess whether cardiac spheroids could regenerate the damaged heart muscles.


在他们的新方法中,研究人员在培养HiPSCs的培养基中,导致它们分化为心肌细胞。在从培养物中仔细提取和净化心脏球体(心脏细胞的三维簇)后,他们向食蟹猕猴(Macaca fascicularis)受损的心脏注射了大约6×10⁷个细胞。他们监测了动物的状况十二周,定期测量心脏功能。在此之后,他们在组织层面分析了猴子的心脏,以评估心脏球体是否可以再生受损的心脏肌肉。


Research Results 研究成果

Prevent Infection

The team confirmed that the hearts of monkeys that received treatment had better left ventricular ejection after four weeks compared to the control group, indicating a superior blood pumping capability. Histological analysis ultimately revealed that the cardiac grafts were mature and properly connected to pre-existing existing tissue, cementing the results of previous observations.


研究小组证实,与对照组相比,接受治疗的猴的心脏在四周后具有更好的左心室射血,这表明其具有更好的血液泵送能力。并且组织学分析最终显示,心脏移植已经成熟,并与先前存在的组织正确连接,巩固了之前观察的结果。


Implication 研究应用


HiPSC-derived cardiac spheroids could potentially serve as an optimal form of cardiomyocyte products for heart regeneration, given their straightforward generation process and effectiveness. The results of this research will help solve the major issue of ventricular arrhythmia that occurs after cell transplantation and will greatly accelerate the realization of cardiac regenerative therapy.


因为生成过程和有效性,HiPSC衍生的心脏球体有可能成为心脏再生心肌细胞产品的最佳形式。这项研究的结果将有助于解决细胞移植后发生的室性心律失常这一主要问题,并将大大加速心脏再生治疗的实现。

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